Notice for depositing Drosophila strains Revised Edition
Kyoto Institute of Technology
Drosophila Genetic Resource Center
Director Masa-Toshi YAMAMOTO
We basically consider operating our Drosophila Genetic Resource Center as "An incubator for your laboratory" as stated at the General Assembly of Japanese Drosophila Research Conferences held in Mishima in 2001, and in Tokyo in 2003. It is difficult to maintain minor strains required only for personal research, however, and we would like to focus on significant strains such as those that are versatile and efficient or mutants of new genes. In fact, our center received various strains from individual research institutions and researchers so far. We would like to make a continued effort to maintain strains into the future. If you would like to deposit any strains, please contact us at jpn-fly@kit.jp.
Conditions for accepting strains
- They should be new mutants.
- They should be efficient strains which are newly developed.
- In the case of transgenic strains, their construct and base sequence should be provided.
- They should be described in academic papers.
- There is no restriction for distribution of strains to any researchers.
- Careful examination is required for the number of transgenic strains and choice for multiple allele and identical construct strains.
NOTE: Even though our center has limited capacity for maintaining strains, our basic principle is to keep strains as much as possible. Please note that received strains are subject to change in accordance with the number of distribution.
NOTE: With respect to deposits to our center, in the case where MTA (Material Transfer Agreement) is required, we would like to deal with it on an individual basis.
Please note the following reminders for distribution of strains:
- Statement of genotype: It is required to state the genotype of each strain since the strain list is based on genotype. As our stocks conform to nomenclature the of FlyBase, please refer to the following website: Genetic nomenclature for Drosophila melanogaster
- Alleles: In the case where the name of allele is not stated in the strain list, please verify it carefully. For instance, if only "w" is written down, please check if it is
w1 or not. If it is unknown, it should be described as w[*]. - Cited references: Please submit publication citations in order to clarify the strain information and place of origin.
- Information of insertion site: Essential for transgenic strains.
- Additional information: It would be convenient for users if details of expression pattern and allele are provided.
- Quarantine: Our center pays special attention to preventing mites and fungi. Please make sure no strains are infected when you deposit them. Moreover, we carry out strict inspection when we receive strains, which will increase the period before we can disclose and distribute strains.
Principles regarding maintenance, management and distribution of strains
Drosophila Genetic Resource Center constitutes the core of the National BioResouce Project (NBRP) "Drosophila" and carries out maintenance, management, and distribution of Drosophila. The NBRP "Drosophila" steering committee is in charge of deliberation concerning the outline of strains. For more detail, please refer to the minutes of the steering committee. If you have any comments, please contact members of the commission. We hope to make improvements based on the Drosophila community's feedbacks.
Our center does not make provisions for depositing stocks at this moment. There are two reasons: For one, researchers are relatively free to share stocks each other, and another is that it is essential for promotion of fundamental research to establish an easy procurement system. Recognizing those situations, our center plans to consider an appropriate response.
Sub-institutes of NBRP "Drosophila" are in charge of the stocks listed below. If you have any inquiries, please contact the person in charge at each sub-institute.
- Mutants of Drosophila melanogaster and RNAi strains: NIG-Fly
Genetic Strains Research Center, National Institute of Genetics (Ryu UEDA: rueda@lab.nig.ac.jp) - Drosophila strains in Japan including Drosophila melanogaster wild type:
EHIME-Fly
Department of Earth Sciences, Faculty of Science, Ehime University (Masayoshi WATADA: watada@mserv.sci.ehime-u.ac.jp - Mutant strains of allied species:
KYORIN-Fly
School of Medicine, Kyorin University (Muneo MATSUDA: matsudam@kyorin-u.ac.jp
