Drosophila Genomics and Genetic Resources

7月13日、米国オーランドMarriott World CenterにおいてNIHワークショップ「Cryopreservation of Drosophila Strains」が開催され、高野が出席しました。これはthe Genetics Society of America (GSA) が企画した The Allied Genetics Conference 2016 に一日先立って開催されたものです。ショウジョウバエ研究者だけでなく、多様な生物の凍結保存を研究、実施している研究者から理論家、工学研究者まで幅広い人材が集まり、有意義な討論が行われました。これからNIHの研究者と外部の Scientific Advisory Board メンバーにより集会レポートが作成されます。レポートはNIHの新規プログラムの立ち上げに繋がるかもしれません。個人的には秋田大学の関さんと知り合うことができました。かれは現在、魚類を中心に凍結保存技術の確立を目指して研究をおこなっています。これからは、ショウジョウバエについても協力してもらえればありがたいです。（TSK）

以下はプログラムです

Purpose and Overview of the Meeting: The goal of this workshop is to evaluate the potential and practicality of using cryopreservation to maintain Drosophila stocks. This workshop will offer a forum for stakeholders to review the needs, current status, limitations or bottlenecks, novel techniques and future directions of cryopreservation in Drosophila. The workshop will be divided into three sessions with short talks from experts and participant discussion.

8:00 - 8:15

Registration

8:15 - 8:20

Introduction and Welcome
Sige Zou (ORIP, NIH)

8:20 - 9:15

Session 1: Overview of Challenges to Cryopreservation in Drosophila and Other Insects
Session Chair: Thomas Kaufman (Indiana Universitty, USA)

8:20-8:40

The Challenges of Drosophila Cryopreservation and the Potential of Frog Aqua-Glyceroporin to Meet the Challenges Hugo Bellen (Baylor College of Medicine, USA)

8:40-9:00

Applied Cryobiology in Managed Insect Systems Joseph Rinehart (USDA-ARS, USA)

9:00-9:15

Q&A

9:15 - 1:00

Session 2: Emerging and Novel Technologies Applicable to Cryopreservation in Drosophila

9:15-10:15

Panel 1 Genetic and Dietary Approaches for Cryopreservation Panel Chair: Pam Geyer (University of Iowa, USA)

9:15-9:30

Insect Freeze Tolerance: Lessons from Nature Brent Sinclair (University of Western Ontario, Canada)

9:30-9:45

A Temperature-Dependent Shift in Dietary Preference Alters the Viable Temperature Range of Drosophila Marko Brankatschk (Max Planck Institute, Germany)

9:45-10:00

Cryopreservation Modeling Enables Rational Protocol Design and Develops Informed Hypotheses: the Past, Present, and Future of Modeling Cryopreservation in Drosophila James Benson (Northern Illinois University, USA)

10:00-10:15

Q&A

10:15-10:30

Break1

10:30-11:30

Panel 2 Cryopreservation via Sperm, Embryos and Larvae
Panel Chair: Brent Sinclair (Western Universtity, Canada)

10:30-10:45

Developing Drosophila IVF Protocols for Preservation of Drosophila Stocks Jianjun Sun (University of Connecticut, USA)

10:45-11:00

How to Preserve Genetic Materials when it is Difficult to Cryopreserve their Embryos Shinsuke Seki (Akita University, Japan)

11:00-11:15

Genetic and physiological approaches to minimising freezing damage: ice-nucleation dynamics in cryoprotected first instar larvae David Gubb (CNRS, France)

11:15-11:30

Q&A

11:30-12:30

Lunch Break1

12:30 - 1:45

Panel 3 Robotics and Instrument Development
Panel Chair: Joseph Rinehart (USDA-ARS, USA)

12:30-12:45

Anhydrous Preservation Methods for Genome Resource Banking Gloria Elliott (University of North Carolina at Charlotte, USA)

12:45-1:00

Similarities in Historical Development and the Approach to Improvement of Vitrification Methods for Human Infertility Treatment and the Preservation of Drosophila Strains Steven F. Mullen (Cook Regentec, USA)

1:00-1:15

The power of Microfluidics to Move Cells and Solutes Mehmet Toner (Harvard Medical School, USA)

1:15-1:30

Technology and Cost Benefits of Automation of Arthropod Embryo Cryopreservation Arun Rajamohan (USDA-ARS, USA)

1:30-1:45

Q&A

1:45 - 2:30

Session 3: Questions/Open Discussion/Recommendations
Session Chairs:
 Mehmet Toner (Harvard Medical School, USA)
 Toshiyuki Takano-Shimizu (Kyoto Stock Center, Japan)

1:45-1:50

Summary of Cryopreservation Technologies
Mehmet Toner and Toshiyuki Takano-Shimizu

1:50-2:30

Open Discussion and Recommendations